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APS Style uses four levels of headings:
level 1
METHODS
level 2
General Experimental Procedures
level 3
Comparison of NADH fluorescence and nitroblue tetrazolium staining in ischemic-reperfused hearts. Coronary occulsion was maintained for 45 min and reperfusion for 14 min. . . .
level 4
ISCHEMIA-REPERFUSION EXPERIMENTS. When reflow was allowed to occur under the latter condition, not only did a much larger number of NADH-deficient myocytes appear . . .
level 3 followed by level 4
Comparison of fluorescences of NADH and propidium iodide in ischemic and ischemic-reperfused myocardium. ISCHEMIA EXPERIMENTS. The dye did not induce any apparent changes . . .
Am J Physiol Heart Circ Physiol 285: H755-H765, 2003.
Level 1 Headings are most often used to label the "Methods", "Results", and "Discussion" sections of the article. "References" and "Disclosures" may also be labeled with level 1 headings, but these headings are inserted by the printer, not the copy editor.
If the author only uses two levels of headings in the manuscript, do not use level 2 headings; skip to Level 3 for all headings under level 1. If the author uses more than two levels of headings, mark headings in the usual sequence.
Exception
AJP - Regu often employs a special section called Perspectives. Perspectives should always be marked as a level 2 heading, even if there are no other level 2 headings in the article.
If the author employs more than four levels of headings, use list style if necessary to denote further divisions in the text.
Headings should not begin with the word "The" -- delete the word if present.
Do not number or letter headings as in outline style.
Exception
Physiological Reviews uses numbered and lettered headings, outline style.
Italicized terms in a level 2 or level 3 heading stay italicized.
The codes used to denote each level of heading are «h1»...«/h1», «h2»...«/h2», «h3»...«/h3», and «h4»...«/h4», respectively. The codes should go at the beginning and end of the heading text, like so:
«h1»MATERIALS AND METHODS«/h1»
Set level 1 headings in all caps.
Do not set level 2 and 3 headings in italics or level 4 headings in small caps. The printer will do this automatically. Level 4 headings should be set in all lowercase at manuscript stage, so that the printer will properly convert them to small caps.
Text immediately following a level 1 or 2 heading does not require a «p» code at the beginning of the paragraph. The fact that it follows a heading indicates to the printer that it should be indented.
«h1»RESULTS«/h1»
In the first set of tests, subjects responded with...
Level 3 and 4 headings should be run into the first line of the paragraph they precede. A paragraph beginning with a level 3 or 4 heading does not require a «p» code. The period at the end of the heading should go inside the closing code. Leave a space between the closing code and the beginning of the paragraph text.
«h3»Whole cell patch-clamp methods.«/h3» The whole cell patch-clamp technique was employed...
If a level 4 heading immediately follows a level 3 heading, run them both into the first line of the paragraph, one after the other, with a space in between.
«h3»Primary cultures.«/h3» «h4»washing medium.«/h4» The medium was supplemented with...
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last edited 07/28/03